DNA

Part:BBa_K2440033

Designed by: Qijie Xu   Group: iGEM17_NUDT_CHINA   (2017-10-22)


miR3476 target sequence

It is the target sequence of miR3476, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR3476 in an Ago2 dependent manner, that is, knockdown of miR3476 was achieved by transfecting cells with miRNA locker.

Ghr-miR3476-5p was firstly found in cotton TIGR Plant1. And its function is still unknown.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.


Reference

1.Enrichment of a set of microRNAs during the cotton fiber development. Kwak PB, Wang QQ, Chen XS, Qiu CX, Yang ZM BMC Genomics. 10:457(2009).

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